The central part of the 5.8 S rRNA is differently arranged in programmed and free human ribosomes Научная публикация
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Biochemical journal
ISSN: 1470-8728 |
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| Вых. Данные | Год: 2005, Том: 387, Номер: 1, Страницы: 139–145 Страниц : DOI: 10.1042/BJ20041450 | ||
| Ключевые слова | human ribosome, oligonucleotide derivative, photo-cross-linking, 5.8 S rRNA, ribosomal protein | ||
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Реферат:
A sequence-specific modification of the human 5.8 S rRNA in isolated 60 S subunits, non-programmed 80 S ribosomes and ribosomes complexed with mRNA and tRNAs was studied with the use of a derivative of the nonaribonucleotide UCUGUGUUU bearing a perfluorophenylazide group on the C-5 atom of the 5′-terminal uridine. Part of the oligonucleotide moiety of the derivative was complementary to the 5.8 S rRNA sequence ACACA in positions 82–86 flanked by two guanines at the 5′-terminus. The target for the cross-linking was identified as nucleotide G89 on the 5.8 S RNA. In addition, several ribosomal proteins were modified by the oligonucleotide derivative bound to the 5.8 S rRNA and proteins L6 and L8 were among them. Application of these results to known cryo-electron microscopy images of eukaryotic 60 S subunits made it possible to suggest that the central part of the 5.8 S rRNA containing the sequence 82–86 and proteins L6 and L8 are located at the base of the L1 stalk of the 60 S subunit. The efficacy of cross-linking in non-programmed 80 S ribosomes was much lower than in isolated 60 S subunits and in programmed 80 S ribosomes. We suggest that the difference in the accessibilities of the central part of the 5.8 S rRNA in the programmed and non-programmed 80 S ribosomes is caused by a conformational switch that seems to be required to dissociate the 80 S ribosomes into the subunits after termination of translation to allow initiation of translation of a new template.
Библиографическая ссылка:
GRAIFER D.
, MOLOTKOV M.
, EREMINA A.
, VEN'YAMINOVA A.
, REPKOVA M.
, KARPOVA G.
The central part of the 5.8 S rRNA is differently arranged in programmed and free human ribosomes
Biochemical journal. 2005. V.387. N1. P.139–145. DOI: 10.1042/BJ20041450 WOS Scopus OpenAlex
The central part of the 5.8 S rRNA is differently arranged in programmed and free human ribosomes
Biochemical journal. 2005. V.387. N1. P.139–145. DOI: 10.1042/BJ20041450 WOS Scopus OpenAlex
Даты:
| Поступила в редакцию: | 25 авг. 2004 г. |
| Принята к публикации: | 5 окт. 2004 г. |
| Опубликована в печати: | 5 нояб. 2004 г. |
| Опубликована online: | 5 нояб. 2004 г. |
Идентификаторы БД:
| Web of science: | WOS:000228431900015 |
| Scopus: | 2-s2.0-17144367651 |
| OpenAlex: | W2071896597 |