Photoinduced cytotoxic activity of a rare ruthenium nitrosyl phenanthroline complex showing NO generation in human cells

Photoinduced cytotoxic activity of a rare ruthenium nitrosyl phenanthroline complex showing NO generation in human cells Научная публикация

Журнал

Dalton Transactions
ISSN: 1477-9234 , E-ISSN: 1477-9226

Вых. Данные

Год: 2024, Том: 53, Номер: 43, Страницы: 17642-17653 Страниц : 12 DOI: 10.1039/d4dt02653e

Авторы

Yakovlev Ivan A. ¹ , Golubeva Julia A . ¹ , Klyushova Lyubov S. ² , Kostin Gennadiy A . ¹ , Mikhailov Artem A. ³

Организации

1	Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 3 Acad. Lavrentiev Avenue, Novosibirsk 630090, Russia.
2	Federal Research Center of Fundamental and Translational Medicine, Timakova Str. 2, Novosibirsk 630060, Russia.
3	Université de Lorraine, CNRS, CRM2, UMR 7036, Nancy 54000, France.

A new nitro-nitrosyl complex [RuNO(Phen)(NO2)2OH] (1) was synthesized and characterized by X-ray diffraction, where Phen = 1,10-phenanthroline. The complex was crystallized in two different modifications without (1) and with a solvent molecule of DMF (1a). The photolysis process together with the determination of the quantum yield of NO release was investigated in acetonitrile solution using a special flow-through system for the simultaneous registration of infrared (IR) and optical absorption (UV-vis) spectra under irradiation with 450 nm light. The quantum yield of photoinduced NO release was 4.0 ± 0.2%. DFT calculations showed that the main contribution to the absorption band at 450 nm is made by the HOMO/HOMO−1 → LUMO transitions, which are represented by the transfer of electron density from the –OH and –NO2 ligands to the orbitals located on the Ru–NO bond. The dark and photoinduced cytotoxicity of the complex was studied against the human breast adenocarcinoma (MCF-7) and lung carcinoma (A549) cell lines and human non-tumor lung fibroblasts (MRC5). The complex shows a low cytotoxicity on MCF-7 cells (ICdark50 = 90.6 ± 6.2 μM and ICirr.50 = 95.3 ± 11.4 μM) and a moderate dark cytotoxicity on A549 and MRC5 cells (ICdark50 = 33.4 ± 2.6 μM and ICdark50 = 62.6 ± 3.1 μM, respectively), which slightly increases after irradiation (ICirr.50 = 21.2 ± 3.3 μM and ICirr.50 = 47.2 ± 2.3 μM, respectively).

Yakovlev I.A. , Golubeva J.A. , Klyushova L.S. , Kostin G.A. , Mikhailov A.A.
Photoinduced cytotoxic activity of a rare ruthenium nitrosyl phenanthroline complex showing NO generation in human cells
Dalton Transactions. 2024. V.53. N43. P.17642-17653. DOI: 10.1039/d4dt02653e WOS Scopus OpenAlex

Поступила в редакцию:	18 сент. 2024 г.
Принята к публикации:	30 сент. 2024 г.
Опубликована online:	8 окт. 2024 г.
Опубликована в печати:	21 нояб. 2024 г.

Web of science:	WOS:001333186000001
Scopus:	2-s2.0-85207360837
OpenAlex:	W4403231927

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